Panc 05.04

The Panc 05.04 cell line is a pancreatic ductal adenocarcinoma (PDAC) model that serves as a valuable resource for studying pancreatic cancer biology and therapeutic responses. Originating from a human pancreatic carcinoma, Panc 05.04 represents a critical tool in preclinical research due to its relevance to one of the most aggressive malignancies with poor patient outcomes. This cell line displays molecular and genomic characteristics that align with the heterogeneous nature of PDAC, including mutations commonly observed in KRAS, TP53, and other driver oncogenes that are prevalent in pancreatic cancer progression.

Panc 05.04 has been extensively profiled as part of large-scale genomic initiatives, such as the Cancer Cell Line Encyclopedia (CCLE), where its gene expression, copy number variations, and drug sensitivity have been characterized. Notably, Panc 05.04 has been assessed for its pharmacological response to various anticancer agents, offering insights into its sensitivity or resistance profiles to chemotherapy and targeted therapies.

As a model system, Panc 05.04 facilitates investigations into the molecular mechanisms underlying PDAC progression, chemoresistance, and tumor microenvironment interactions. It also provides a platform for testing emerging targeted therapies and immunotherapeutic strategies aimed at overcoming the refractory nature of pancreatic cancers.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.