HEK293-PSMA

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HEK293-PSMA cells are human embryonic kidney 293 (HEK293) cells engineered to stably express human prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (FOLH1/GCPII). PSMA is a type II transmembrane glycoprotein with enzymatic folate hydrolase and carboxypeptidase activity that is highly expressed in prostate cancer, particularly in advanced, metastatic, and castration-resistant disease. In addition to prostate malignancies, PSMA expression has also been observed in the neovasculature of various solid tumors. Because of its strong tumor-associated expression and accessible extracellular domain, PSMA has become a major target for diagnostic imaging, radioligand therapy, antibody-based therapeutics, and engineered immune cell approaches.

HEK293-PSMA cells are widely used in oncology research and therapeutic development for characterization of PSMA-targeted monoclonal antibodies, antibody-drug conjugates, radiopharmaceuticals, bispecific T-cell engagers, CAR-T cell therapies, and small-molecule inhibitors. The stable recombinant expression system enables quantitative analysis of ligand binding, receptor occupancy, antigen density, internalization kinetics, and target-dependent cytotoxicity. These cells are particularly valuable for evaluating PSMA-directed imaging probes and radioligand platforms, as PSMA undergoes efficient internalization following ligand engagement. Additional applications include flow cytometry assay development, uptake studies, reporter assays, high-throughput screening, and validation of targeted delivery systems for prostate cancer therapeutics.

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere for at least 24 hours.

For best attachment and viability after thawing the cells, we recommend using Collagen-coated flasks or plates for the initial seeding after cryo-recovery. Collagen coating is not required for subsequent routine culture of the cells.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.