LS174T

The LS147T cell line is a variant of LS-180, both of which are derived from a Duke's type B adenocarcinoma of the colon in a 58-year-old White female patient. The original LS-180 line was established by culturing the minced tumor tissue for 10 months. LS-147T, along with its parent line, is notable for its expression of multiple oncogenes including myc, myb, ras, and fos, while being negative for others like sis, abl, and ros. This line also expresses high levels of carcinoembryonic antigen (CEA), interleukin 6 (IL-6), and interleukin 10 (IL-10), which are important markers and potential targets in colorectal cancer research.

These cells exhibit several key characteristics of colonic epithelial cells, including abundant microvilli and intracytoplasmic mucin vacuoles, which are features typically associated with secretory cells in the colonic mucosa. Electron microscopy studies have confirmed these structural details, further supporting their origin and differentiation status. Importantly, LS-147T cells have been shown to be tumorigenic in immunodeprived mice, consistently producing tumors when inoculated subcutaneously at high cell densities, thus affirming their malignant potential.

Moreover, the LS-147T cell line is particularly valuable in studies focusing on the molecular and immunological aspects of colorectal cancer. It has been reported that this line is easier to subculture compared to its parent line, LS-180, making it a more practical choice for long-term studies. The robust production of CEA by these cells, which is significantly higher than that of other established lines like HT-29, makes LS-147T a critical model for understanding tumor marker dynamics and exploring targeted therapies in colorectal cancer.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.