SW-579

SW-579 is a human thyroid squamous cell carcinoma cell line, commonly used in cancer research to study thyroid cancer progression and invasiveness. This cell line has been particularly valuable in research exploring the role of matrix metalloproteinases (MMPs) and integrins in cancer cell invasion. Studies involving SW-579 have demonstrated that bone sialoprotein (BSP) significantly enhances the invasiveness of these cells by forming a trimolecular complex with MMP-2 and integrin αvβ3. This complex promotes cancer cell movement through extracellular matrices, mimicking the invasive behavior of metastatic cancers.

In vitro experiments using a modified Boyden chamber invasion assay have shown that treating SW-579 cells with BSP increased their invasiveness by approximately 10-fold compared to untreated controls. This enhanced invasiveness was found to be mediated by MMP-2 and integrin αvβ3, as blocking either the integrin or MMP-2 significantly reduced the effect. These findings highlight the critical role of MMPs and integrins in the metastatic potential of thyroid cancers, making SW-579 a useful model for studying targeted therapies aimed at disrupting these pathways.

Moreover, the involvement of BSP in SW-579 cell invasiveness suggests potential therapeutic targets for inhibiting metastasis in thyroid carcinoma. By interfering with the formation of the BSP-MMP-2-integrin αvβ3 complex, researchers may be able to reduce the invasiveness of these cancer cells, offering a promising approach to limiting the spread of thyroid cancer in patients.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.