NCI-H1792

NCI-H1792 是一种源自成年患者肺腺癌的人类非小细胞肺癌（NSCLC）细胞系。该细胞系在癌症研究中得到了广泛应用，特别是在针对肺肿瘤发生、遗传异常及药物敏感性分析的研究中。 该细胞系具有上皮样形态，在培养中可形成贴壁单层。其被纳入“癌症细胞系百科全书”（CCLE）等大规模数据集，使得对其进行广泛的基因组和蛋白质组分析成为可能，从而促进了与其他肺癌模型的比较分析。

在基因组层面，NCI-H1792 表现出非小细胞肺癌（NSCLC）中常见的多种分子改变。已知该细胞系携带 KRAS 突变——这是肺腺癌中常见的致癌驱动基因，会导致 MAPK 信号传导异常。 该细胞系还经过了蛋白质组学研究分析，其蛋白质表达谱为了解信号通路的依赖性和脆弱性提供了见解。蛋白质组学数据突显了其在理解KRAS突变型癌症的通路调控及药物靶点验证方面的实用价值。这些数据集还强调了该细胞系属于KRAS驱动型癌症的一个亚型，该亚型表现出独特的代谢和信号传导特征。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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