SU-DHL-8

SU-DHL-8是一种源自成人患者的人类弥漫性大B细胞淋巴瘤（DLBCL）细胞系。该细胞系代表DLBCL中的活化B细胞样（ABC）亚型，其特征为NF-κB信号通路的持续激活，且相较于生发中心B细胞样（GCB）亚型，通常预后更差。 形态学上，SU-DHL-8细胞在悬浮培养中形成大型松散粘附聚集体，符合B细胞淋巴瘤表型特征。

分子特征分析显示，SU-DHL-8携带与ABC-DLBCL相关的常见突变，包括影响B细胞受体（BCR）和NF-κB信号通路的改变。通过新一代测序和表达分析进行的基因组测序，已识别出JAK/STAT和BCL2相关抗凋亡信号通路等通路的活性升高。 该细胞系还参与了多项大规模药物基因组学研究及癌症模型库项目，被用于探索药物敏感性（特别是激酶抑制剂和蛋白酶体靶向剂）。这些特性使SU-DHL-8成为研究ABC型DLBCL分子发病机制及治疗靶点的代表性模型。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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