DT40

The DT40 cell line is derived from chicken (Gallus gallus) and is primarily used as a model for studying B-cell biology and immunology. Originating from an avian leukosis virus-induced bursal lymphoma, DT40 cells are unique due to their high rate of homologous recombination, making them particularly valuable for gene targeting studies. This characteristic allows for efficient and precise genetic modifications, facilitating research in various fields including DNA repair, chromosomal dynamics, and gene function. DT40 cells exhibit rapid growth and a stable karyotype, which are advantageous traits for experimental reproducibility and consistency. They are extensively used to study the mechanisms of immunoglobulin gene conversion and diversification, contributing significantly to our understanding of the adaptive immune system. Moreover, DT40 cells are employed in pharmacological research to screen for potential therapeutic compounds and to analyze cellular responses to DNA damage. In addition to their applications in immunology and genetic research, DT40 cells are also instrumental in investigating cellular signaling pathways. They provide a robust system for dissecting the roles of various proteins and genes in signal transduction, apoptosis, and cell cycle regulation. The ease of genetic manipulation in DT40 cells, combined with their biological relevance, continues to make them a crucial tool in biomedical research. However, it is essential to note that findings in DT40 cells should be validated in mammalian systems due to species-specific differences.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.