IHH-4

The IHH-4 cell line is derived from papillary thyroid carcinoma (PTC), the most prevalent form of thyroid cancer, which frequently exhibits aggressive characteristics including invasion and metastasis. IHH-4 has been utilized in numerous studies focused on elucidating the molecular mechanisms underpinning PTC progression. This cell line is particularly noted for its role in studies investigating epithelial-mesenchymal transition (EMT), a process that enhances the invasive potential of cancer cells. For example, it has been shown that IHH-4 cells, along with other PTC lines, express elevated levels of matrix metalloproteinase-9 (MMP-9), a protease that plays a critical role in degrading the extracellular matrix and facilitating tumor invasion and metastasis. Inhibition of MMP-9 in IHH-4 cells was found to reduce EMT markers and hinder cell migration and invasion.

Research involving the IHH-4 cell line has also examined the role of transcription factors such as T cell factor 4 (TCF4) and long non-coding RNAs (lncRNAs) in PTC. Studies have highlighted that TCF4 is overexpressed in IHH-4 cells and can regulate the expression of the lncRNA HCP5, which in turn modulates several microRNAs related to tumor progression. Knockdown of TCF4 in IHH-4 cells was shown to decrease cell proliferation and invasion, suggesting that TCF4 is a pivotal regulator of oncogenic pathways in PTC.

Overall, IHH-4 serves as a valuable model for studying molecular and cellular pathways related to thyroid cancer, particularly those that drive cancer cell invasion, metastasis, and resistance to therapies. The insights gained from research using IHH-4 contribute to the development of potential therapeutic strategies for combating aggressive thyroid cancers.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.