U2932

The U-2932 cell line is a diffuse large B-cell lymphoma (DLBCL) model that was established from a patient who developed secondary DLBCL following treatment for Hodgkin lymphoma (HL). The cell line exhibits morphological characteristics typical of DLBCL and has been shown to form colonies in nude mice, confirming its tumorigenic potential. U-2932 is derived from ascitic fluid and maintains a B-cell phenotype with a somatically hypermutated immunoglobulin heavy chain (IgH) gene rearrangement. Notably, the cell line is Epstein-Barr virus (EBV) negative, distinguishing it from some other lymphoma-derived cell lines.

Genomic characterization of U-2932 reveals a complex karyotype, including high-level amplifications at chromosomal bands 18q21 and 3q27, regions associated with oncogenic drivers such as BCL-2 and BCL-6, both of which are overexpressed in this cell line. Additionally, the cell line carries a mutation in the tumor suppressor gene p53 at amino acid position 176, with immunohistochemical analysis confirming p53 overexpression. These genetic alterations suggest a role in both tumor progression and potential resistance to chemotherapy.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.