Human Mesenchymal Stem Cells, Bone Marrow (HMSC-BM)

Human Mesenchymal Stem Cells derived from Bone Marrow (HMSC-BM) represent a robust and versatile tool for in vitro research. These multipotent mesenchymal stromal cells (MSCs) possess the unique ability to self-renew and differentiate into a broad spectrum of cell types, including adipocytes, osteoblasts, and chondrocytes. The potential of HMSC-BM to differentiate into these three key lineages has been well-documented, making them invaluable for studies focused on regenerative medicine, tissue engineering, and cellular differentiation pathways. These MSCs are cultivated under stringent conditions, ensuring their multipotency and high viability post-thaw.

One of the distinguishing features of HMSC-BM compared to MSCs derived from other sources, such as adipose tissue or umbilical cord, is their superior capacity for osteogenic differentiation. This makes them particularly useful in bone biology and orthopedic research, where understanding the molecular mechanisms governing bone formation and repair is crucial. Additionally, HMSC-BM exhibit a robust immunomodulatory profile, which makes them an excellent model for studying immune interactions and inflammatory responses. These unique characteristics also position HMSC-BM as a preferred choice for preclinical studies exploring bone marrow microenvironment, hematopoiesis, and the pathophysiology of bone marrow-related diseases.

Each cryovial of HMSC-BM contains a minimum of 1 x 10⁶ cells, with viability rates ranging between 92% to 95%, as determined by the Trypan Blue dye exclusion test. These cells are derived from bone marrow collected from healthy adult donors, all of whom have provided informed consent. To ensure the highest standards, each batch undergoes rigorous quality control testing to assess cell identification, purity, potency, and viability. This thorough testing guarantees that the MSCs meet strict criteria, making them suitable for a wide range of research applications, including cell biology studies, drug discovery, and the investigation of cellular responses to different stimuli. These cells are not intended for therapeutic or in vivo applications, and their use is confined to research purposes in a controlled laboratory environment.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.