MDCC-MSB1

The MDCC-MSB1 cell line is a lymphoblastoid cell line derived from a chicken with Marek's disease, a highly contagious viral disease caused by Marek's disease virus (MDV), which belongs to the herpesvirus family. These cells are extensively used in veterinary virology and immunology research to study the pathogenesis of MDV, as well as in the development and evaluation of vaccines against this disease. The MDCC-MSB1 cell line exhibits characteristics typical of lymphoid cells, such as the expression of specific surface markers and cytokine production, which are crucial for understanding the immune response to MDV infection.

In addition to its role in MDV research, the MDCC-MSB1 cell line is valuable for studying general mechanisms of oncogenesis and viral replication in avian species. The cells are known for their robust growth in suspension culture, making them convenient for large-scale production and experimental manipulation. Researchers utilize this cell line to investigate the molecular interactions between MDV and its host, to identify viral and host factors involved in disease progression, and to screen potential antiviral compounds. Overall, the MDCC-MSB1 cell line is a vital tool in both basic and applied avian virology research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.