Farage

The Farage cell line originates from a B lymphocyte derived from an adult female diagnosed with non-Hodgkin's B-cell lymphoma. This cell line is particularly valuable in immunological studies due to its unique characteristics and reactions to various stimuli. Farage cells grow in suspension and are notable for not expressing surface or cytoplasmic immunoglobulins, highlighting their utility in studies focused on immune response without the interference of these proteins.

When treated with interleukin-4 (IL-4), Farage cells exhibit an increase in the expression of several markers including CD23, CD54, and CD58, while showing a reduction in CD21, CD22, and CD38 levels. This modulation of surface markers suggests IL-4’s role in influencing B-cell behavior and provides a useful model for exploring the signaling pathways and regulatory mechanisms in B-cells. Moreover, the response to phorbol 12-myristate 13-acetate (PMA) treatment, which results in the down-regulation of CD21 and CD23, further supports its application in studying kinase-driven signaling in B-cells.

The absence of terminal deoxynucleotidyl transferase (TdT) and recombination activating genes (RAG-1 and RAG-2) in Farage cells confirms their classification as mature B-cells rather than pre-B cells. This aspect is crucial for research targeting the mature stages of B-cell development or function. Additionally, the presence of Epstein-Barr virus (EBV) in these cells can be leveraged in studies investigating viral interactions with host cellular mechanisms, particularly in the context of oncogenic processes in lymphocytes.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.