SF126

The SF126 cell line is a human glioblastoma cell line, widely utilized in research on brain tumors, particularly in studies exploring the molecular mechanisms of glioblastoma and its response to various treatments. Derived from a patient with glioblastoma multiforme, SF126 cells are known for their aggressive growth and invasive behavior, typical of glioblastomas, making them a crucial model for investigating therapeutic strategies and understanding tumor biology. One of the notable features of SF126 is its use in exploring both apoptosis (programmed cell death) and autophagy, as these processes are central to cancer cell survival and resistance to treatment.

SF126 has been extensively studied for its interactions with p53, a tumor suppressor gene frequently mutated in cancers. In SF126, researchers have investigated the effects of wild-type and mutant p53 on cell death mechanisms. It was found that p53 induces both apoptosis and autophagy, with autophagic cell death playing a significant role in p53-dependent cell death. This has implications for therapies targeting autophagic pathways, which may enhance the efficacy of treatments aimed at inducing tumor cell death. Additionally, studies have shown that manipulating autophagy can influence the overall tumor response to p53 activation, offering potential therapeutic angles for glioblastoma treatment.

Further research on SF126 has explored its binding properties with opioid peptides, such as β-endorphins, revealing specific binding sites for these molecules. This has provided insights into how glioblastoma cells might interact with endogenous hormones and signaling molecules in the brain, further underscoring the complexity of glioblastoma biology and potential novel therapeutic targets.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.