RH-35

H4-II-E（亦稱為 RH-35）細胞系是 Reuber H-35 大鼠肝癌細胞系的衍生物。該細胞系源自一隻雄性 ACI 大鼠因接觸化學致癌物 N-2-芴乙醯胺而誘發的肝腫瘤。 當移植至 ACI 大鼠體內時，H4-II-E 細胞會形成生長迅速的腫瘤，其組織學特徵具有低分化肝癌的典型表現。 該細胞對芳香烴羥化酶（AHH）活性的誘導特別敏感，使其成為研究多環芳香烴和二噁英類化合物酶促反應的可靠系統。

鑑於 H4-II-E 細胞的克隆形成能力以及檢測治療後長期細胞存活的能力，它們亦可用作研究細胞對致癌物和輻射反應的模型。 其應用範圍更延伸至探索酶誘導機制、異物代謝以及肝臟特異性毒理學。這些特性使 H4-II-E 成為癌症研究與毒理學篩選中不可或缺的工具。

37°C，5% CO_₂，加濕環境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

透過基於 PCR 的檢測及基於發光原理的支原體檢測方法，排除支原體污染的可能性。

為確保無細菌、真菌或酵母菌污染，每日均會對細胞培養物進行目視檢查。

若需長期保存，請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前，可將試管暫時存放於 −80 °C 環境中，作為短期過渡步驟。

冷凍保存的細胞系會以乾冰冷藏，並裝於經驗證的隔熱包裝中，包裝內含有足夠的冷媒，以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後，請立即檢查容器，並盡快將試管轉移至適當的儲存場所。

在 Cytion，我們對產品的品質與存活率提供保障。 我們保證，自本公司設施出貨後 60 天內，我們的細胞在培養開始時仍具存活能力。 我們保證所有材料均符合 分析證明書及／或產品說明書中所載的規格。

詳情請參閱我們的 《保固聲明》。