16HBE140-

16HBE140 细胞系源自人类支气管上皮细胞，是研究呼吸道上皮细胞的关键。这些细胞保留了原代支气管上皮细胞的几个关键特征，包括形成紧密连接的能力、表达特征性标记物以及表现出典型的上皮形态。它们被广泛用于呼吸系统疾病、药物运输和毒理学研究，为了解支气管上皮细胞在各种条件下的行为提供了可靠的体外模型。

16HBE140 细胞的重要应用之一是研究囊性纤维化（CF），这是一种影响呼吸系统的遗传疾病。这些细胞表达囊性纤维化跨膜传导调节因子（CFTR）蛋白，是研究囊性纤维化病理生理学和筛选潜在治疗药物的重要工具。此外，16HBE140 细胞还可用于气道炎症研究，因为它们对促炎细胞因子和污染物有反应，有助于了解哮喘和慢性阻塞性肺病 (COPD) 等慢性呼吸系统疾病。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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