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Colo-320DM 细胞
Tissue
结肠，杜克C型
Disease
大肠腺癌
Synonyms
COLO_320DM, COLO-320-DM, COLO #320DM, COLO320/DM, COLO320-DM, COLO320DM, Colo320DM, COLO320 DM, COLO 320 DM, COLO 320 (DM), Colorado 320 Double Minutes

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Colo-320DM 细胞

Cell line · Product number 300153

The COLO-320DM cell line is a human colorectal adenocarcinoma cell line established from the metastatic site of a 55-year-old Caucasian female. This cell ...

US$395.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

COLO-320DM 细胞系是一种人类结直肠腺癌细胞系，从一名 55 岁的白种女性的转移部位建立。该细胞系具有独特的特征，对研究结直肠癌转移和化疗药物的作用具有重要意义。值得注意的是，它的癌胚抗原（CEA）表达量很高，而癌胚抗原是用于监测和诊断结直肠癌的重要生物标志物。

COLO-320DM 细胞具有上皮样形态的粘附性。它们经常被用于研究结直肠癌进展和转移的细胞和分子机制。此外，由于它们的生长模式和遗传稳定性始终如一，因此是研究癌细胞生物学、药物反应和结直肠癌相关基因表达的体外实验的可靠模型。

这些细胞也是基因研究特别感兴趣的对象，尤其是与转移和化疗反应相关的通路。研究人员利用 COLO-320DM 探索信号通路、细胞对缺氧的反应以及癌细胞与肿瘤微环境之间的相互作用。该细胞系在开发针对结直肠癌转移机制的治疗策略方面发挥了重要作用。

Tissue
结肠，杜克C型
Disease
大肠腺癌
Synonyms
  • COLO_320DM
  • COLO-320-DM
  • COLO #320DM
  • COLO320/DM
  • COLO320-DM
  • COLO320DM
  • Colo320DM
  • COLO320 DM
  • COLO 320 DM
  • COLO 320 (DM)
  • Colorado 320 Double Minutes

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
圆形和折射
Tumourigenic
是，在裸鼠身上

04Identifiers

Citation
COLO-320DM（Cytion 目录号 300153）
NCBI TaxID
9606
Cellosaurus
CVCL_0219

05Expression and susceptibility

Isoenzymes
PGM1,1, PGM3, 2, G6PD, B, PEP-D, 1, 6PGD, A, ES-D, 1
Products
羟色胺、去甲肾上腺素、肾上腺素、促肾上腺皮质激素（ACTH）、甲状旁腺激素

06Handling and culture

Culture medium
Ham's F12，w：1.0 mM 稳定谷氨酰胺，w：1.0 mM 丙酮酸钠，w：1.1 g/L NaHCO3（Cytion 文章编号 820600a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基，用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS，T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞，T25 烧瓶用 1-2 毫升，T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟，使其分离。孵育后，用 10 毫升培养基轻轻混合细胞使其重悬，然后用 300xg 离心 3 分钟。弃去上清液，用新鲜培养基重悬细胞，并将其转移到已装有新鲜培养基的新烧瓶中。
Seeding density
1 × 10⁴ 个细胞/平方厘米
Fluid renewal
每 3 至 5 天
Post-thaw recovery
解冻后，将细胞以5×10⁴个细胞/平方厘米的密度接种于培养皿中，使细胞从冷冻过程中恢复并至少贴壁24小时。
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
55 岁
Ethnicity
高加索人