769-P

The 769-P cell line is a human renal cell carcinoma (RCC) cell line that was derived from a nephrectomy specimen of a 63-year-old female patient with renal cell adenocarcinoma in 1975. It is widely used in renal cell cancer research, particularly clear cell renal cell carcinoma (ccRCC), which is the most common and lethal form of kidney cancer in adults.

The 769-P cell line retains many characteristics of primary RCC and harbors several mutations that are relevant to renal cell carcinoma. They exhibit a loss of function in the von Hippel-Lindau (VHL) tumor suppressor gene, which is an important kidney cancer gene in ccRCC that can activate various oncogenic pathways including angiogenesis, cell proliferation, and metabolic reprogramming.

The 769-P cell line is used to understand the molecular mechanisms of kidney cancer pathogenesis, explore the efficacy of anticancer drugs, and investigate the mechanisms of drug resistance. These cells are particularly useful for studying the response to tyrosine kinase inhibitors (TKIs), which are a class of targeted therapies used in the treatment of RCC and RCC subtypes.

The 769-P renal cancer cell line is further used to investigate the role of the tumor microenvironment in kidney cancer and to study cellular processes like apoptosis, cell cycle regulation, and metastatic potential. Their responsiveness to hypoxic conditions makes them suitable for research into how ccRCC adapts to and thrives in low-oxygen environments found within solid tumors.

In summary, the 769-P cell line and other RCC cell lines are indispensable tools in renal carcinoma research, providing insights into the pathogenesis of ccRCC, drug efficacy, and resistance mechanisms.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.