A204

A204 cells are human epithelial cells derived from the muscles of a 1-year-old female patient with rhabdomyosarcoma. With applications in 3D cell culture and tumorigenic properties, A-204 cells provide an opportunity for studying tumour biology and potential therapeutic interventions. Derived from muscle tissue, A-204 cells closely resemble the outer layer of cells found in organs and tissues.

The A204 cell line is characterized by its aggressive undifferentiated phenotype, making it a valuable model for investigating the molecular mechanisms of tumorigenesis and metastasis in soft tissue sarcomas.

The presence of specific isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3, in A-204 cells provides insight into their metabolic characteristics. These isoenzymes may play a role in understanding cellular processes involved in cancer progression and treatment response.

These cells exhibit robust growth in vitro and have been used to study cell proliferation, apoptosis, and drug resistance mechanisms. The A204 cell line is also instrumental in the evaluation of new chemotherapeutic agents and in understanding the interaction between rhabdomyosarcoma cells and therapeutic compounds.

This cell line serves as an essential tool for cancer researchers aiming to develop more effective treatments for sarcomas and other related malignancies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.