CA46

The CA46 cell line is a human cell line derived from a Burkitt's lymphoma, which is a type of non-Hodgkin's lymphoma. This cell line exhibits characteristics typical of a transformed B lymphocyte lineage and was originally established from the malignant cells of a 39-year-old male. CA46 cells are noteworthy for their study in oncology research, particularly in understanding the Epstein-Barr virus (EBV) negative Burkitt’s lymphoma pathogenesis and the underlying molecular biology of B-cell differentiation and transformation.

Scientifically, CA46 cells have been instrumental in the study of gene expression related to B-cell development and malignancy. They are EBV-negative, which allows researchers to investigate tumor characteristics and behaviors without the influence of EBV, a common confounder in many lymphoid malignancies. The cell line also provides a useful tool for examining the efficacy of therapeutic agents and the mechanisms of drug resistance in lymphoma, contributing to the development of targeted therapies in hematologic cancers.

In research settings, CA46 cells have been used to assess cytotoxic responses to chemotherapeutic agents and to explore signal transduction pathways involved in B-cell proliferation and apoptosis. Their genomic stability and susceptibility to genetic manipulation further enable their use in molecular biology and genetic studies related to cancer research and therapy development.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.