EOMA

The EOMA cell line, also known as EOMA endothelial cells, is derived from a spontaneously arising hemangioendothelioma in a mouse. This cell line is extensively used in research to study angiogenesis, the process of new blood vessel formation, which is critical in both normal physiological processes and in pathological conditions such as cancer, diabetic retinopathy, and rheumatoid arthritis. EOMA cells are characterized by their endothelial origin, displaying properties typical of endothelial cells, including the formation of capillary-like structures in vitro.

Researchers utilize the EOMA cell line to investigate the molecular and cellular mechanisms underlying angiogenesis. This includes studies on the role of various growth factors, signaling pathways, and the extracellular matrix in endothelial cell proliferation, migration, and tube formation. EOMA cells are particularly valuable in evaluating the effects of anti-angiogenic compounds, which are used in the treatment of cancer and other diseases involving abnormal blood vessel growth. These cells are also used in gene expression studies and in the development of therapeutic strategies targeting angiogenesis.

In addition to angiogenesis research, EOMA cells serve as a model for studying hemangioendothelioma, a rare vascular tumor, providing insights into tumor biology and the identification of potential therapeutic targets. By offering a reliable and reproducible in vitro system, the EOMA cell line significantly contributes to the understanding of vascular biology and the development of treatments for angiogenesis-related diseases.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.