3T6-Swiss albino

The 3T6-Swiss albino cell line originates from the tissue of Swiss albino mice, specifically developed for a broad range of virological and oncological research purposes. This fibroblast cell line is known for its susceptibility to various viruses, including murine sarcoma viruses, making it an invaluable tool in the study of viral oncogenesis and the transformational properties of oncogenes in a controlled environment. The robustness of 3T6-Swiss albino cells in culture allows for detailed genetic manipulation and analysis, facilitating advanced genetic studies that seek to understand the intricacies of cancer progression and viral infection mechanisms.

In addition to its applications in virology, the 3T6-Swiss albino cell line is frequently used in pharmacological research. Its responsiveness to pharmaceutical agents makes it a suitable model for drug screening and toxicity testing. Researchers utilize these cells to examine the cellular responses to new compounds, evaluating their efficacy and safety before proceeding to more complex in vivo studies. The genetic stability of the 3T6-Swiss albino cell line over multiple passages supports consistent experimental results, which is crucial for the development of reliable therapeutic strategies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.