CADO-ES1

The CADO-ES1 cell line was established from a malignant pleural effusion taken from a 19-year-old female patient diagnosed with Ewing's sarcoma, primarily located in the right buttock with multiple lung metastases. This cell line provides a valuable tool for research in sarcoma biology, particularly in studying the metastatic processes associated with Ewing's sarcoma. As a disease primarily affecting children and young adults, Ewing's sarcoma is characterized by small round cells that are highly malignant, often exhibiting aggressive behavior and poor prognosis, particularly when metastatic.

Uniquely, CADO-ES1 cells exhibit several critical features valuable for in-depth cancer research. They are heterotransplantable, meaning they can be transplanted into a different species (e.g., mice), which is vital for in vivo studies. This capacity makes them a robust model for studying tumor growth and metastasis in a controlled, yet biologically relevant system. Additionally, these cells have shown the ability to grow independently of anchorage, a characteristic typical of many cancerous cells which allows them to thrive without adhering to the extracellular matrix. Furthermore, CADO-ES1 cells can differentiate neurally in response to cyclic AMP (cAMP), providing a unique perspective into the cellular behaviors influenced by signaling pathways in cancer progression and differentiation.

This combination of features makes CADO-ES1 a significant model for not only understanding the pathology of Ewing's sarcoma but also for the development and testing of targeted therapies that might inhibit the growth and spread of similar cancers. Research utilizing this cell line can contribute to a deeper understanding of cancer cell behavior, metastatic mechanisms, and potential therapeutic targets in sarcomas.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.