CHO-IL2RA

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CHO-IL2RA cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human interleukin-2 receptor alpha (IL-2Rα; CD25/IL2RA), a high-affinity cytokine receptor subunit involved in regulation of T-cell activation and immune homeostasis. CD25 forms part of the heterotrimeric IL-2 receptor complex together with IL-2Rβ (CD122) and the common gamma chain (CD132), enabling high-affinity binding of interleukin-2 and activation of downstream JAK/STAT signaling pathways. Physiologically, CD25 is highly expressed on activated T lymphocytes and regulatory T cells (Tregs), and aberrant expression has also been reported in several hematologic malignancies and inflammatory disorders.

CHO-IL2RA cells are widely used in immunology and therapeutic development workflows for characterization of anti-CD25 monoclonal antibodies, cytokine-based therapeutics, bispecific antibodies, and engineered immune cell targeting strategies. The stable recombinant expression system enables quantitative assessment of ligand binding, receptor occupancy, antibody affinity, and receptor internalization. These cells are also valuable for flow cytometry assay development, potency testing, cell-based binding assays, and high-throughput screening applications involving IL-2 pathway modulation. In addition, CHO-IL2RA models can support studies examining selective targeting of activated T cells or regulatory T-cell-associated mechanisms in autoimmunity, transplantation, and cancer immunotherapy.

For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)

For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.