H22

The H22 cell line is a murine hepatocellular carcinoma cell line derived from liver tumor cells. These cells are commonly used in cancer research to study liver cancer mechanisms, therapeutic interventions, and drug efficacy. H22 cells exhibit typical characteristics of hepatocellular carcinoma, including rapid proliferation, resistance to apoptosis, and the ability to form tumors when injected into suitable animal models. This makes them a valuable tool for in vivo studies aiming to understand tumor growth, metastasis, and the tumor microenvironment in liver cancer.

One of the significant advantages of the H22 cell line is its use in immunotherapy research. Since the cells are derived from a murine model, they are particularly useful for studying the interactions between cancer cells and the immune system in a controlled environment. Researchers utilize H22 cells to evaluate the efficacy of various immunotherapeutic agents, including checkpoint inhibitors and cancer vaccines. Additionally, H22 cells are employed in the investigation of liver-specific metabolic pathways and the role of genetic mutations in hepatocellular carcinoma progression.

Overall, the H22 cell line serves as a robust model for hepatocellular carcinoma, providing insights into cancer biology and aiding in the development of novel therapeutic strategies. Its relevance to both in vitro and in vivo studies underscores its importance in the field of cancer research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.