NRK-52E

The NRK-52E cell line, derived from the normal kidney of a rat, is an epithelioid cell line representing proximal tubular epithelial cells. This cell line is widely used in nephrology research, especially for studies on renal physiology, toxicology, and pathophysiology. NRK-52E cells display characteristic epithelial morphology with tight junctions, making them suitable for in vitro modeling of renal tubular function and barrier integrity.

NRK-52E cells have been instrumental in studying mechanisms of apoptosis, cellular repair, and ion transport. For instance, the cell line has been used to investigate the effects of okadaic acid, a protein phosphatase inhibitor, revealing its role in inducing apoptotic pathways involving chromatin condensation, calcium influx, and mitochondrial changes. These studies have provided insights into the regulation of renal cell death and survival mechanisms during injury or disease.

Furthermore, NRK-52E cells have been used to assess renal epithelial ion transport and barrier properties under various experimental setups, such as microfluidic systems that mimic physiological flow conditions. This includes research on sodium chloride reabsorption and transepithelial electrical resistance, which are critical for understanding electrolyte and water balance in renal physiology. These characteristics make NRK-52E a robust model for exploring renal tubular cell biology and therapeutic interventions in kidney diseases.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.