SNU-638

SNU-638 细胞系是从一名男性胃癌患者的腹水中提取的人类胃癌模型。它的分化程度很低，脱落细胞极少，在体外以混合模式生长，密度不均，对培养基质的附着力很差。细胞保持圆形至椭圆形轮廓，核质比低，微绒毛发育有限。这些特点反映了与侵袭性胃癌表型相关的常见特征，使该品系适合研究分化较差的胃腺癌。

在分子水平上，SNU-638 不携带*c-Ki-ras*基因突变，但表达高水平的肿瘤相关标志物，如 CA 19-9 和组织多肽抗原（TPA），而缺乏甲胎蛋白（AFP）表达。它还携带*TP53*基因突变，这种突变在胃癌中经常出现，在肿瘤发生过程中起着核心作用。基因组分析表明，SNU-638缺乏MET扩增或过表达，因此被归类为MET阴性，对MET信号通路的依赖性极低。这种分子特征使 SNU-638 成为针对 MET 或评估 MET 抑制剂在胃癌中疗效的研究中一个有价值的对照细胞系。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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