C33A

C-33 A cells originate from the cervical tissue of a 66-year-old Caucasian female diagnosed with uterine cancer. This cell line is characterized by a unique genetic alteration in the TP53 gene, where a point mutation at codon 273 results in an arginine to cysteine substitution, leading to elevated expression of the p53 protein. This mutation plays a critical role in the pathophysiology of the cells, influencing their growth properties and tumorigenic potential.

Notably, C-33 A cells are confirmed to be tumorigenic. When introduced into immunodeficient nude mice, these cells have the capability to form undifferentiated carcinomas, which highlights their utility in cancer research, particularly in studies aimed at understanding the mechanisms of tumor initiation and progression in cervical cancer. Furthermore, these cells are negative for both DNA and RNA of human papillomavirus (HPV), distinguishing them from many other cervical cancer cell lines which often carry HPV integrations. This aspect makes C-33 A cells particularly valuable for studying cervical cancer that develops independently of HPV infection, offering insights into alternative pathways of carcinogenesis.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.