A498

A498 cells are a human renal cell carcinoma cell line derived from the kidney tissue of a 58-year-old Caucasian male. These cells are extensively used in research related to kidney cancer, particularly for studying clear cell renal cell carcinoma, which is the most common type of kidney cancer in adults.

The A498 cell line is characterized by its epithelial-like morphology and has been a valuable model for investigating the molecular and cellular mechanisms of renal carcinogenesis. These cells exhibit several features typical of kidney cancer, including alterations in the expression of genes involved in cell cycle regulation, apoptosis, and angiogenesis.

A498 cells are particularly useful for examining the metabolic pathways altered in kidney cancer, as they display a distinct metabolic profile that includes changes in lipid and glucose metabolism. This aspect makes them suitable for metabolic targeting studies, which explore how altering metabolic pathways can inhibit tumor growth.

Furthermore, A498 cells are employed in drug discovery and toxicology studies to test the efficacy of new chemotherapeutic agents and targeted therapies. They are also used to study the response of renal cancer cells to hypoxic conditions-a common feature of solid tumors that significantly influences tumor behavior and treatment response.

Overall, the A498 cell line serves as an essential tool in renal cancer research, facilitating the development of more effective therapeutic strategies and enhancing our understanding of kidney cancer biology.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.