HCT-15

HCT-15 cells are derived from the adenocarcinoma of the colon of a 44-year-old Caucasian male. This cell line, developed in the early 1970s, is widely utilized in the field of cancer research, especially for exploring the biology and treatment of colorectal cancer.

Morphologically, HCT-15 cells are characterized by an epithelial-like appearance with a tendency to grow both as a monolayer and in clusters, displaying significant cellular heterogeneity. This feature mirrors the varied cellular environments found in solid tumors, making HCT-15 a valuable model for studying tumor dynamics and cellular interactions within the tumor microenvironment.

Genotypically, HCT-15 cells exhibit a hyperdiploid karyotype with multiple chromosomal aberrations, typical of many colorectal cancers. These include mutations in key oncogenes and tumor suppressor genes, such as mutations in the KRAS gene and deletions affecting the p53 pathway, which are implicated in the pathogenesis and progression of colorectal cancer. These genetic traits make HCT-15 cells a crucial tool for investigating genetic and molecular mechanisms associated with cancer progression, metastasis, and resistance to therapies.

The broad use of HCT-15 cells in research has led to significant insights into the molecular pathways involved in colorectal cancer, enhancing our understanding of disease mechanisms and aiding in the development of targeted therapies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.