L6

The L6 cell line is a well-established model derived from rat skeletal muscle tissue. These cells are notable for their ability to differentiate into myotubes, making them a valuable tool for studying muscle development, regeneration, and physiology. L6 cells exhibit robust proliferative capacity and are commonly used in research focusing on muscle cell biology, including studies on muscle protein synthesis, hypertrophy, and atrophy. The differentiation process in L6 cells can be induced under specific culture conditions, leading to the formation of multinucleated myotubes that closely mimic the characteristics of mature skeletal muscle fibers.

In addition to their applications in muscle physiology research, L6 cells are also employed in metabolic studies, particularly those involving glucose uptake and insulin signaling pathways. These cells express insulin receptors and can be used to investigate the molecular mechanisms underlying insulin resistance and diabetes. The L6 cell line's responsiveness to various metabolic stimuli makes it an ideal model for exploring the effects of different treatments or genetic modifications on muscle metabolism. Overall, L6 cells provide a versatile and reliable platform for advancing our understanding of muscle biology and metabolic diseases.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.