LP-1

The LP-1 cell line is a well-established human multiple myeloma cell line derived from a patient with multiple myeloma. It is characterized by its t(4;14)(p16;q32) translocation, which results in the dysregulated expression of fibroblast growth factor receptor 3 (FGFR3). This genetic aberration is a hallmark of a subset of multiple myeloma cases and is associated with the pathogenesis and progression of the disease. LP-1 cells express a functional FGFR3, which, when activated, can engage the MAP kinase signaling pathway, promoting cell proliferation and survival. Notably, LP-1 carries a non-activating F384L mutation in the FGFR3 gene, distinguishing it from other myeloma cell lines with activating mutations of FGFR3.

LP-1 cells are useful for studying the role of FGFR3 in multiple myeloma, particularly in the context of non-activating mutations. Research has shown that in multiple myeloma, FGFR3 mutations and other common oncogenic mutations, such as those in the Ras family, are typically mutually exclusive, suggesting that these mutations may contribute to tumorigenesis through similar or overlapping pathways. This makes LP-1 an invaluable model for exploring the molecular mechanisms underlying multiple myeloma and for testing targeted therapies aimed at the FGFR3 pathway.

In addition to its relevance in FGFR3-related studies, LP-1 is also significant in research focused on the broader aspects of myeloma biology, including the role of cytokines like interleukin-6 (IL-6) in cell survival and proliferation. This cell line has been instrumental in studies that investigate the interactions between myeloma cells and their bone marrow microenvironment, as well as in the development of novel therapeutic strategies aimed at disrupting these interactions to control disease progression.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.