HepG2.2.15

The HepG2.2.15 cell line is a derivative of the HepG2 cell line, which originates from a human hepatoblastoma, a type of liver cancer. These cells are particularly noteworthy for their ability to stably express hepatitis B virus (HBV) particles, making them invaluable in the study of HBV biology and the development of antiviral drugs. HepG2.2.15 cells maintain many of the characteristics of hepatocytes, including the production of proteins such as albumin and alpha-fetoprotein, which are critical for liver function. Additionally, they possess a polygonal shape and form tight clusters, resembling liver tissue structure.

One of the primary uses of the HepG2.2.15 cell line is in researching HBV replication and pathogenesis. These cells are transfected with the HBV genome, leading to the continuous production of viral particles. This feature makes them an ideal model for studying the lifecycle of HBV and the effects of various antiviral agents. Researchers utilize HepG2.2.15 cells to screen for potential therapeutic compounds, investigate viral entry and replication mechanisms, and understand the host's immune response to HBV infection. The cell line's ability to produce HBV also allows for the study of viral mutations and resistance patterns, which is crucial for developing effective treatments.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.