Human Subcutaneous Pre-Adipocytes
Cell line · Product number 300697
Human Subcutaneous Pre-Adipocytes are primary preadipocytes isolated from human subcutaneous adipose tissue. They can be expanded and differentiated into mat...
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- Human Subcutaneous Pre-AdipocytesCell line€800.00
Recommended (freshest lot)
01General information
- Description
- Human Subcutaneous Pre-Adipocytes are primary preadipocytes isolated from human subcutaneous adipose tissue. They can be expanded and differentiated into mature adipocytes, serving as a model for adipogenesis, metabolism, obesity, and diabetes research.
- Tissue
- Adipose tissue (subcutaneous)
- Disease
- Normal
- Applications
- Adipogenesis and differentiation studies; obesity, metabolism and diabetes research
02Classification
- Organism
- Human
- Growth properties
- Adherent
- Biosafety level
- 1
03Characteristics
- Morphology
- Fibroblast-like (preadipocyte)
- Cell type
- Preadipocyte
- Doubling time
- Approximately 48-72 hours
04Identifiers
- Citation
- Human Subcutaneous Pre-Adipocytes (Cytion catalog number 300697)
- NCBI TaxID
- 9606
05Handling and culture
- Culture medium
- Preadipocyte Growth Medium
- Incubation atmosphere
37°C, 5% CO2, humidified atmosphere.
- Supplements
- Growth supplements; serum (FBS) as per medium
- Dissociation reagent
- Trypsin-EDTA or Accutase
- Subculturing
- At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium.
- Split ratio
- 1:2 to 1:3
- Seeding density
- 5 x 10^3 cells/cm2
- Fluid renewal
- Every 2-3 days
- Thawing and culturing
Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.
Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.
For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.
Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.
Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.
Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.
Follow the procedure described under Post-Thaw Recovery
06Quality control and cryopreservation
- Freeze medium
- As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
- Storage conditions
For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.
- Shipping conditions
Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
07Legal and safety
- Safety precautions
- When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
- Warranty
At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.
For full details, please refer to our Warranty Statement.
- Material Transfer Agreement required
- Yes