MET-5A

The MET-5A cell line is derived from mesothelial cells of the pleura in a human adult and is often used in research related to mesothelioma, a type of cancer affecting the mesothelial lining of the lungs, abdomen, and heart. These cells are crucial for studying the biology, pathogenesis, and treatment of mesothelioma, particularly in understanding how environmental factors such as asbestos exposure lead to the development of this cancer. MET-5A cells are also used to explore the mechanisms of cellular transformation, tumor progression, and the cellular responses to various chemotherapeutic agents.

MET-5A cells exhibit a typical epithelial morphology and retain characteristics of normal mesothelial cells, including the expression of mesothelial markers such as cytokeratin and vimentin. These cells are responsive to inflammatory stimuli and can be used to study the inflammatory processes involved in mesothelioma pathogenesis. Researchers employ MET-5A cells to investigate the genetic and molecular alterations associated with mesothelioma, as well as to test the efficacy and toxicity of potential therapeutic compounds. The relevance of MET-5A cells in modeling mesothelial cell biology and their role in mesothelioma research makes them an essential tool for advancing our understanding and treatment of this aggressive cancer.

37°C, 5% CO₂, humidified atmosphere.

Supplement the medium with 15% FBS, 15 mM HEPES, 1% ITS+

The trace elements at the following final concentrations:

H2SeO3 0.3869 mg/L (Selenious acid)

MnCl2×4H20 0.0198 mg/L (Manganese chloride)

Na2SiO3×9H20 14.2100 mg/L (Sodium silicate)

(NH4)6Mo7O24×4H20 0.1236 mg/L (Ammonium molybdate)

NH4VO3 0.0585 mg/L (Ammonium vanadate)

NiSO4×6H20 0.0131 mg/L (Nickle sulfate)

SnCl2×2H20 0.0113 mg/L (Tin Chloride)

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.