TE10

The TE10 cell line is part of the TE-series, a collection of esophageal cancer models established in Japan, specifically from a well-differentiated squamous cell carcinoma of the esophagus. TE10 cells are notable for their epithelial morphology and growth in monolayer culture. These cells were derived from a primary lesion without prior radiation exposure, making them representative of untreated tumor biology.

TE10 exhibits several key genetic alterations linked to esophageal cancer progression. These include amplification and overexpression of the c-erbB gene, co-amplification of the int-2 and hst-1 proto-oncogenes, and dysregulation of growth factors such as TGF-α and EGF-R. These molecular features make TE10 a valuable model for investigating pathways involved in tumor growth, such as the epidermal growth factor receptor (EGFR) signaling axis. In addition, the line has been used to study responses to targeted therapies and chemotherapeutic agents, shedding light on mechanisms of drug resistance.

TE10 cells are also frequently used in invasion and migration assays due to their capacity to form colonies in soft agar and exhibit anchorage-independent growth. These characteristics highlight their utility in exploring the molecular drivers of metastasis. The cell line’s consistent performance in experiments focused on tumor biology and pharmacology makes it an important tool for translational cancer research, particularly for developing therapeutic strategies aimed at squamous cell carcinoma of the esophagus.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.