Human Mesenchymal Stem Cells, Chorion Villi

Human Mesenchymal Stem Cells (MSCs) derived from chorion villi represent a highly versatile population of multipotent stromal cells capable of differentiating into multiple lineages, including adipocytes, osteoblasts, and chondrocytes. These cells are isolated from the chorionic villi, a part of the placenta that plays a critical role in maternal-fetal exchange. Chorion villi are unique in that they are composed of both fetal and maternal tissues, providing a distinct microenvironment that contributes to the robust self-renewing and differentiation capabilities of the MSCs derived from this source. The MSCs from chorion villi exhibit a more primitive phenotype compared to MSCs derived from adult tissues, often displaying a higher proliferation rate and broader differentiation potential. These characteristics make them particularly valuable for research in regenerative medicine, tissue engineering, and disease modeling.

These MSCs have been rigorously demonstrated in vitro to differentiate into adipocytes, osteoblasts, and chondrocytes when cultured in lineage-specific differentiation media, underscoring their potential for applications in tissue regeneration and disease modeling. The unique origin of these cells from the chorionic villi imparts them with specific immunomodulatory properties, which may differ from MSCs derived from other sources such as bone marrow or adipose tissue. This distinction is crucial for studies focusing on immune-related conditions or developing allogeneic cell therapies.

MSCs are cryopreserved at early passages in a specialized cryomedium, ensuring their viability and functionality post-thaw. Each cryovial contains a minimum of 1 x 10⁶ cells with a viability rate ranging between 92% and 95%, as determined by the Trypan Blue dye exclusion test. These cells are sourced from healthy donors who have provided informed consent, ensuring ethical collection practices. Each batch undergoes stringent quality control assessments, including thorough testing for cell identification, purity, potency, and viability. These measures guarantee that the cultured MSCs are of high quality and appropriate for research applications, excluding therapeutic or in vivo use.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.