AR42J

AR42J cells are a rat pancreatic tumor cell line derived from azaserine-induced tumors in rats. They are widely used as a model for studying pancreatic exocrine cell functions, pancreatitis, and pancreatic cancer research. AR42J cells exhibit acinar-like characteristics, making them particularly valuable for investigating the physiology and pathology of pancreatic acinar cells.

One of the defining features of AR42J cells is their ability to differentiate into cell types exhibiting more pronounced pancreatic exocrine functions when treated with various agents, such as dexamethasone or activators of protein kinase C. Upon differentiation, these cells produce and secrete digestive enzymes, including amylase, lipase, and chymotrypsin, mimicking the enzyme secretion profile of normal pancreatic acinar cells.

AR42J cells are also used to explore the mechanisms of acute pancreatitis. They respond to stimuli like cerulein, a cholecystokinin analog, which can induce a condition in the cells that resembles acute pancreatitis, characterized by enzyme overproduction, oxidative stress, and inflammatory responses. This makes AR42J cells a useful tool for testing potential therapeutic interventions for pancreatitis.

Furthermore, the AR42J cell line is utilized in research focused on pancreatic cancer, particularly for studies on tumorigenesis and the malignant transformation of acinar cells. They are instrumental in examining the effects of oncogenes, tumor suppressor genes, and growth factors on the development and progression of pancreatic cancer.

Overall, AR42J cells provide a versatile and dynamic model system for advancing our understanding of pancreatic diseases and for the development of new therapeutic strategies targeting these conditions.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.