PC-3M

The PC-3M cell line is a metastatic variant derived from the human prostate adenocarcinoma PC-3 cell line, originally isolated from a bone metastasis of a prostate cancer patient. PC-3M was established to better model the metastatic potential of prostate cancer. This cell line exhibits enhanced migratory and invasive capabilities compared to its parental counterpart, making it a critical tool in studying the molecular mechanisms of metastasis and evaluating therapeutic interventions targeting metastatic prostate cancer.

PC-3M cells have been used in various in vitro and in vivo studies to investigate tumor progression and therapeutic resistance mechanisms. They have shown adaptability to diverse culture conditions and exhibit robust growth both in standard culture and in animal models. Notably, the PC-3M line has been widely applied in xenograft studies, where it demonstrates the ability to form tumors and metastasize efficiently, replicating key characteristics of advanced-stage prostate cancer. This makes it an invaluable model for testing anti-metastatic agents and elucidating pathways that drive metastatic dissemination.

In addition to its metastatic properties, PC-3M has been utilized to explore interactions between tumor cells and the microenvironment, including the role of stromal cells and extracellular matrix components in promoting cancer progression. The cell line also expresses biomarkers relevant to prostate cancer, such as prostate-specific antigen (PSA), and is amenable to genomic and proteomic profiling, enabling researchers to investigate molecular pathways and identify potential therapeutic targets.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.