A549/DDP

The A549/DDP cell line is a drug-resistant variant of the A549 cell line, which itself is a model of human alveolar basal epithelial adenocarcinoma. This variant has been specifically selected for its resistance to cisplatin (DDP), a common chemotherapy drug used in the treatment of various cancers, including lung cancer. The development of the A549/DDP cell line enables researchers to study the mechanisms underlying chemoresistance, which is a major challenge in cancer therapy.

In research, the A549/DDP cell line is utilized to investigate the biochemical pathways involved in cisplatin resistance. This includes the exploration of changes in gene expression, protein function, and cellular metabolism that confer resistance to cisplatin. The cell line is also valuable in the screening of new drugs or drug combinations that can overcome resistance, providing insights that are crucial for the development of more effective therapeutic strategies against lung cancer.

Moreover, studies using the A549/DDP cell line contribute to a better understanding of the molecular basis of lung cancer progression and metastasis in the context of chemoresistance. This cell line serves as a critical tool for translational research, bridging experimental findings to potential clinical applications in oncology.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.