OE33

OE33 is a human oesophageal adenocarcinoma cell line derived from a primary tumor associated with Barrett’s oesophagus. This cell line is extensively used in research to explore the molecular biology and therapeutic responses of oesophageal adenocarcinoma, particularly in the context of Barrett’s oesophagus progression. OE33 provides a relevant model for understanding the pathogenesis of this cancer, which has been increasing in incidence and is associated with poor prognosis in advanced stages.

OE33 cells display epithelial morphology and grow adherently under standard culture conditions. The cell line is characterized by molecular features typical of oesophageal adenocarcinoma, including mutations and dysregulated pathways involved in tumorigenesis, such as p53 and HER2/neu signaling. OE33 is particularly useful for investigating cellular responses to therapeutic interventions targeting HER2/neu and other pathways implicated in cancer progression and resistance. This cell line also serves as a tool for studying the interactions between tumor cells and their microenvironment, as well as mechanisms of metastasis and immune evasion.

In preclinical research, OE33 is widely employed in drug screening to evaluate the efficacy of chemotherapy, targeted therapies, and novel treatment combinations aimed at improving outcomes for oesophageal adenocarcinoma. Additionally, the cell line is used in xenograft models to assess tumorigenicity and therapeutic responses in vivo. OE33’s clinical relevance and molecular characteristics make it an invaluable resource for advancing our understanding of oesophageal adenocarcinoma and developing effective treatments for this aggressive disease.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.