SK-N-AS

The SK-N-AS cell line is derived from a neuroblastoma of a human child and is extensively used in neuro-oncology research. Neuroblastoma is a type of cancer that arises from neural crest cells and predominantly affects children. SK-N-AS cells provide a valuable model for studying the biology and treatment of neuroblastoma, particularly in understanding the molecular mechanisms driving tumor development and progression. This cell line is characterized by its relatively undifferentiated state, which makes it useful for examining the pathways involved in neuronal differentiation and malignancy.

SK-N-AS cells exhibit an adherent growth pattern and possess a neuroblastic morphology. They express various markers associated with neural crest cells and neuroblastoma, including neuron-specific enolase (NSE) and chromogranin A. Researchers utilize SK-N-AS cells to investigate the genetic and epigenetic changes associated with neuroblastoma, such as MYCN amplification and ALK mutations. These cells are also employed in high-throughput drug screening and preclinical testing of novel chemotherapeutic agents and targeted therapies. Additionally, SK-N-AS cells are used to study the mechanisms of resistance to conventional therapies and to develop strategies to overcome such resistance. The relevance of SK-N-AS cells in neuroblastoma research underscores their importance in advancing our understanding of this aggressive childhood cancer and in improving therapeutic approaches for affected patients.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.