LoVo

The LOVO cell line, derived from a grade IV Dukes' type C colon adenocarcinoma, is characterized by mutations in the adenomatous polyposis coli (APC) gene, Kirsten rat sarcoma viral oncogene homolog (KRAS), and tumor protein p53 (TP53). These genetic features are instrumental in studying the molecular basis of colorectal cancer progression, metastasis, and drug resistance mechanisms.

LoVo cells serve as a critical model for screening anti-cancer compounds and by understanding how cancer cells like LoVo develop resistance, researchers can design more effective therapies. LoVo cells are also employed in molecular biology studies to explore signaling pathways that regulate cancer cell growth, survival, and metastasis.

In the context of human colon cancer and colorectal cancer cell lines, LoVo cells offer insights into the mechanisms of tumor growth and the process of metastasis, particularly node metastasis, and the tumor microenvironment driving cancer progression. The use of LoVo colon cancer cells, especially in lovo xenograft models, allows researchers to study cancer cell dynamics and metastatic potential.

Deep sequencing and gene expression analysis in LoVo cells have shed light into the specific genes and their roles in colorectal cancer cells. This research has has highlighted the importance of integrins, such as integrin β1, in cancer cell migration and invasion, and the regulation of key molecules like MMP2 in signaling pathways contributing to the understanding of cancer cell lines' invasive properties.

LoVo cells, as a model system in colorectal cancer cell lines, play a pivotal role in advancing our understanding of the molecular aspects of cancer, from gene and protein expression to the intricacies of tumor growth and metastasis.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.