MDA-MB-435S

Disclaimer: The cell line in question has been identified as problematic due to contamination issues. Specifically, the parent cell line (MDA-MB-435) has been shown to be a derivative of the M14 cell line.

The MDA-MB-435S cell line is a widely utilized model in cancer research, originally thought to be derived from a breast cancer metastasis. These cells exhibit characteristics typical of highly aggressive cancer cells, including a rapid proliferation rate, resistance to apoptosis, and the ability to invade surrounding tissues. Due to these traits, MDA-MB-435S cells are frequently used in studies investigating cancer metastasis, drug resistance mechanisms, and the molecular underpinnings of aggressive tumor behavior.

Interestingly, subsequent molecular and genetic analyses have revealed that MDA-MB-435S cells share a closer genetic profile with melanoma rather than breast cancer, raising significant implications for their use in research. Despite this controversy, they remain a valuable model for studying metastatic processes and testing potential therapeutic agents, particularly those targeting mechanisms common to both breast cancer and melanoma. Researchers are advised to consider these genetic findings when interpreting results obtained from studies involving MDA-MB-435S cells.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.