TOV-112D

TOV-112D is a human endometrioid ovarian carcinoma cell line established from the primary ovarian tumor of a 42-year-old Caucasian female patient. The line grows as an adherent monolayer. TOV-112D harbors a TP53 p.Ser37Ala (homozygous) substitution in the transactivation domain, a homozygous PTEN frameshift mutation (p.Leu639fs), and a TP53 p.Arg175His mutation. This combination of TP53 and PTEN alterations makes TOV-112D a relevant model for studying endometrioid-type ovarian carcinoma, which frequently harbors concurrent TP53, PTEN, and CTNNB1 mutations. The doubling time is approximately 22–29 hours.

TOV-112D is applicable in studies of endometrioid ovarian carcinoma biology, TP53 mutant phenotype, PTEN loss and PI3K/Akt pathway activation, drug sensitivity evaluation (carboplatin, paclitaxel, PARP inhibitors), and gynecological oncology research. It is suitable for in vitro pharmacological assays and xenograft models in immunocompromised hosts for preclinical ovarian cancer drug evaluation.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.