RAJI

Raji cells are a line of lymphoblast-like cells established by R.J.V. Pulvertaft in 1963 from Burkitt's lymphoma. These cells are widely used in immunology research due to their high expression of human CD19, which acts as a co-receptor and decreases the threshold for antigen B-cell receptor (BCR) stimulation. Raji cells are non-adherent and grow in suspension as free-floating individuals or doublets.

The doubling time of these cells is 23.2 hours, and they are relatively small in diameter with a diameter range of 5-8 μm. Some characteristics of Raji cells include a lack of differentiation, as they form large aggregations of hundreds of individual cells. These cells are diploid and have a stable karyotype within the male diploid stemline of 46.

Additionally, Raji cells are partially resistant to poliovirus and vesicular stomatitis viruses. Human CD19 is highly expressed by Raji cells and has been identified as a clinical target for anti-hCD19-CD3 bis-specific antibodies in non-Hodgkin's B cell lymphoma. BCMA expression has also been identified in the Raji Burkitt lymphoma cell line and primary lymphoma, making it an important area of research for immunologists.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.