喇嘛-84 细胞

LAMA-84 是一种人体细胞系，提取自处于爆发期的慢性髓性白血病（CML）患者的外周血。这种细胞系的特点是存在费城染色体，导致 BCR-ABL 融合基因，这是 CML 的标志。众所周知，BCR-ABL 致癌基因能增强酪氨酸激酶的活性，从而促进各种信号通路，导致细胞不受控制地增殖并抵抗细胞凋亡。因此，LAMA-84 细胞是研究 CML 进展分子机制和评估酪氨酸激酶抑制剂 (TKI) 临床前疗效的宝贵模型。

在研究中，LAMA-84 被广泛用于了解 CML 的生物学特性，尤其是耐药性和疾病演变方面。涉及该细胞系的研究有助于阐明细胞对不同代 TKIs（如伊马替尼、达沙替尼和尼洛替尼）的反应。此外，LAMA-84 还有助于研究旨在克服 TKI 耐药性的新治疗策略，包括测试针对受 BCR-ABL 融合蛋白协同影响的其他信号通路的联合疗法。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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