HEK293 EBNA 细胞

HEK293 EBNA 细胞系是原始 HEK293 细胞系的衍生物，而原始 HEK293 细胞系本身是由组织培养的人类胚胎肾细胞衍生而来。这种特殊的亚系被设计成能稳定表达爱泼斯坦-巴氏病毒核抗原-1（EBNA-1）。EBNA-1 的表达允许携带 EBV 复制源的质粒进行外显子复制，这使得 HEK293 EBNA 细胞在生产重组蛋白和进行涉及外显子载体的基因表达研究时尤为重要。

HEK293 EBNA 细胞保留了母体 HEK293 细胞的许多特性，包括对细胞培养塑料的粘附性和在标准哺乳动物细胞培养基中的稳健生长。EBNA-1 的加入扩大了它们在研究和生物技术应用中的用途，因为它增强了细胞繁殖具有 EBV 质粒复制起源的质粒的能力。这一特性对于生产稳定、高产的重组蛋白至关重要，而这对于研究目的和工业规模生产都是必不可少的。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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