HCC-LM3

The HCC-LM3 cell line is an established model for studying hepatocellular carcinoma (HCC), particularly due to its high metastatic potential. This cell line has been instrumental in uncovering mechanisms related to tumor proliferation, migration, and treatment resistance. Research on HCC-LM3 cells has revealed their involvement in exploring drug responses and the molecular pathways influencing cancer aggressiveness. For example, circular RNA circMRPS35 has been shown to play an oncogenic role in HCC-LM3, promoting cell proliferation, migration, invasion, and chemoresistance, particularly to cisplatin. Mechanistically, circMRPS35 functions by sponging microRNA-148a-3p, leading to the upregulation of Syntaxin 3 (STX3), which modulates phosphatase and tensin homolog (PTEN) stability through ubiquitination and degradation.

In addition, studies have identified significant metabolic shifts in HCC-LM3 cells that correlate with tumor growth and survival. This cell line, along with other HCC models, demonstrates marked alterations in glucose and lipid metabolism, which support rapid tumor proliferation and are considered hallmarks of liver cancer. Research employing single-cell RNA sequencing has illuminated how metabolic heterogeneity within hepatocyte subpopulations impacts prognosis and therapeutic outcomes. Notably, metabolic pathway analyses in HCC-LM3 have been essential for identifying potential biomarkers and therapeutic targets for improved clinical strategies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.