PANC 08.13

The PANC 08.13 cell line, also known as PL9, is a pancreatic cancer cell model derived from adenocarcinoma. It has been included in studies focused on understanding genomic variations, including allelic loss, homozygous deletions, and broader chromosomal instability. This cell line is part of an extensive set of pancreatic cancer models used to analyze somatic alterations in cancer cells, particularly in the absence of matched normal tissues, making it ideal for high-resolution genetic mapping.

In a study utilizing Affymetrix 100K SNP arrays, PANC 08.13 was examined alongside 25 other pancreatic cancer cell lines. These arrays allowed for high-density analysis of single nucleotide polymorphisms (SNPs) and revealed the allelic status across the genome without requiring matched normal samples. Such studies help identify critical regions of allelic loss (LOH), gene deletions, and chromosomal recombination events, which are frequent in pancreatic cancer cells.

PANC 08.13’s genetic characterization includes frequent mutations in driver genes typically associated with pancreatic cancer, such as KRAS, TP53, CDKN2A, and SMAD4. These genetic defects are hallmarks of pancreatic ductal adenocarcinoma and contribute to uncontrolled cellular proliferation, disrupted cell cycle regulation, and defective apoptosis mechanisms. Studies leveraging cell lines like PANC 08.13 allow for the development of targeted therapies and the identification of novel therapeutic vulnerabilities in pancreatic cancer.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.