A172

A-172 (A172 or A-172 MG) is a significant cell line used in neuroscience research. It originates from the brain tissue of a 53-year-old male with glioblastoma, a type of brain cancer. These cells adhere and spread on the surface of culture dishes, with a karyotype of n = 80 (80 chromosomes). A-172 cells are hypertriploid, exhibiting over 20 marker chromosomes. They have been shown to be non-tumorigenic in anti-thymocyte serum treated NIH Swiss mice. A-172 cells have a gene expression profile that highlights their mesenchymal lineage and involvement in angiogenesis.

They express genes related to mesenchymal markers (CD90, CD105, fibroblast activation protein, tenascin C) and angiogenesis inducers (VEGF, FGF2 (b), TGFb1, thrombospondin-1). Comparisons with the T98G cell line reveal differences in morphology and surface marker expression. Both cell lines show high expression of a2 smooth muscle actin. Changing the fetal serum concentration in the culture medium affects the proportion of cells expressing specific surface antigens, such as CD73 and CD105.

A-172 and T98G cell lines accurately represent glioblastomas, providing valuable tools for studying this brain tumor. Their gene expression profiles and morphological features allow for investigations into the molecular mechanisms underlying glioblastoma development and progression. Researchers can utilize A-172 cells to gain insights into glioblastoma biology and potentially identify new therapeutic targets for this devastating disease.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.